| Pos | Rank | Heat | Bib | Athlete | Mark | Details | Reaction Time | |
|---|---|---|---|---|---|---|---|---|
| 1 | 1 | 2 | 300 | NED | 51.48  | Q | 0.180 | |
| 2 | 1 | 5 | 110 | BAH | 51.74 SB | Q | 0.127 | |
| 3 | 1 | 1 | 245 | IRL | 51.75  | Q | 0.174 | |
| 4 | 2 | 5 | 273 | JAM | 51.86  | Q | 0.139 | |
| 5 | 2 | 1 | 303 | NED | 51.96  | Q | 0.141 | |
| 6 | 3 | 5 | 291 | LTU | 52.11  | q | 0.212 | |
| 7 | 1 | 4 | 319 | POL | 52.22  | Q | 0.187 | |
| 8 | 4 | 5 | 150 | CUB | 52.25 SB | q | 0.162 | |
| 9 | 2 | 4 | 233 | GUY | 52.34  | Q | 0.128 | |
| 10 | 1 | 3 | 323 | POL | 52.37  | Q | 0.260 | |
| 11 | 3 | 4 | 117 | BEL | 52.51 SB | 0.212 | ||
| 12 | 2 | 2 | 215 | GBR | 52.53  | Q | 0.190 | |
| 13 | 3 | 1 | 114 | BAR | 52.65  | 0.166 | ||
| 14 | 3 | 2 | 378 | USA | 52.72  | 0.189 | ||
| 15 | 4 | 4 | 388 | USA | 52.78  | 0.198 | ||
| 16 | 4 | 1 | 272 | JAM | 52.89  | 0.143 | ||
| 17 | 2 | 3 | 208 | GBR | 52.93  | Q | 0.157 | |
| 18 | 3 | 3 | 162 | CZE | 52.94  | 0.194 | ||
| 19 | 5 | 1 | 326 | POR | 53.01  | 0.189 | ||
| 20 | 6 | 1 | 288 | LAT | 53.05 SB | 0.189 | ||
| 21 | 4 | 2 | 159 | CZE | 53.05  | 0.168 | ||
| 22 | 5 | 4 | 177 | ESP | 53.13  | 0.138 | ||
| 23 | 5 | 5 | 343 | SRB | 53.36  | 0.179 | ||
| 24 | 4 | 3 | 242 | IRL | 53.47  | 0.153 | ||
| 25 | 6 | 4 | 232 | GRE | 53.62 SB | 0.203 | ||
| 26 | 5 | 3 | 111 | BAH | 54.03  | 0.186 | ||
| 27 | 5 | 2 | 142 | CAN | 54.65  | 0.169 | ||
| 28 | 6 | 5 | 366 | TKS | 56.20  | 0.188 |